Disputas: Guozhen He

Guozhen He disputerer for doktorgraden i anvendte mikro- og nanosystemer ved Fakultet for teknologi, naturvitenskap og maritime fag.

27 Jun

Praktisk informasjon

  • Dato: 27 juni 2023
  • Tid: kl. 09.30 - 15.30
  • Sted: Vestfold, auditorium A1-39 (Holmestrand) og Zoom
  • Last ned kalenderfil

    • Lenke til digital deltakelse 

    Program

    Kl. 09.30: Prøveforelesing: Integrated biosensing in organ-on-a-chip devices

    Kl. 12.00:  Disputas: Point of Care COPD Diagnostics based on Paper-based Assay and Biofluid Sensing

     

    Bedømmingskomité

    • Første opponent: Førsteamanuensis Graca Maria H. Minas, University of Minho
    • Andre opponent: Professor Truls Norby, Universitetet i Oslo
    • Tredje opponent og administrator: Professor Lars Hoff, Universitetet i Sørøst-Norge

     

    Veiledere

    • Hovedveileder: Professor Tao Dong, Universitetet i Sørøst-Norge
    • Biveileder: Professor Per Alfred Øhlckers, Universitetet i Sørøst-Norge, og dr. Zhaochu Yang, Sensovann AS
Har du spørsmål?

Guozhen He skal forsvare avhandlingen sin for graden philosophiae doctor (ph.d.) ved Universitetet i Sørøst-Norge.

Portrett av Guozhen Zhe

Han har fulgt doktorgradsprogrammet anvendte mikro- og nanosystemer ved Fakultet for teknologi, naturvitenskap og maritime fag ved campus Vestfold.

Alle interesserte ønskes velkommen til prøveforelesning og disputas på campus eller digitalt.

Sammendrag

Chronic obstructive pulmonary disease (COPD) has always been the one of the top four major death causes during the last ten years. During the era of covid-19 pandemic, COPD patients faced higher mortality rates than ever before. Inefficiency in COPD diagnosis and exacerbation monitoring leads to tremendous medical costs and economic burden. Decentralized point-of-care (PoC) diagnostics based on disposable paper-based biosensors emerge as a cost-effective approach, which requires sensitive detection of COPD biomarkers in human saliva and blood as a prerequisite. When testing biological samples, it also needs to consider matrix effects resulted from samples and handling protocols. Biofouling is the most noticeable and deleterious effect of untreated saliva or serum that happens simultaneously on most interfaces and results in blocking and passivation of the sensing surface with non-specific adsorbed proteins.

This work has studied optical and electrochemical detection of COPD biomarker in human saliva by lateral flow assays (LFAs). It has also concluded a solution to practically follow progression of COPD stages with a set of chosen salivary biomarkers and their detection by LFAs as a cost-effective approach. To mitigate the “hook” effect in one-step sandwich LFA, this work introduced a strategy of controlled release of detection antibody via sucrose treatment of the LFAs. To overcome biofouling from non-specific protein adsorption, tungsten trioxide nanosheets with engineered oxygen vacancies were studied as electrode coating with a hypothesis that oxygen vacancies in the surface would affect adhesion of biofouling proteins. Tungsten trioxide rich in surface oxygen vacancies exhibited significantly 95% less adsorption from selected proteins and lower toxicity to human umbilical vein endothelial cells than its counterpart with no oxygen vacancies.